Method for extracting infant saliva internal genome DNA applied to whole genome sequencing

The invention discloses a method for extracting infant saliva internal genome DNA applied to whole genome sequencing. The method is characterized by comprising the following steps of: pretreating, cracking, purifying, precipitating, washing and dissolving. According to the method, the operation is s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	BAO YONGGANG, SHI SONGCHUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a method for extracting infant saliva internal genome DNA applied to whole genome sequencing. The method is characterized by comprising the following steps of: pretreating, cracking, purifying, precipitating, washing and dissolving. According to the method, the operation is safe, easy and convenient, the flux is high, the cost is low and a human body can not be injured and can not feel pain. The extracted genome DNA has high purity and high content, has the fragment being more than 23kb and completely meets the requirements of constructing a whole genome sequencing library and operating a general experiment of molecular biology.