Method of determining the protease cathepsin B in a biological sample

Method of determining the protease cathepsin B in a biological sample

Method of determining the potentially available activity of cathepsin B in a biological sample, including the activity of the active form of cathepsin B, the activable form of cathepsin B from the proform procathepsin B which is present in the sample and the activable form of cathepsin B which is pr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: MAUK HANNS JOERG, MEIER HANS JOERG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Method of determining the potentially available activity of cathepsin B in a biological sample, including the activity of the active form of cathepsin B, the activable form of cathepsin B from the proform procathepsin B which is present in the sample and the activable form of cathepsin B which is present in the sample in a form in which it is inhibited in respect of its protease activity by protease inhibitor, where procathepsin B present in the sample is converted into the active form cathepsin B, the content of free cathepsin-B-protease inhibitor present in the sample is reduced, or the inhibitor function of free cathepsin-B-protease inhibitor present in the sample is blocked and the protease inhibitor is removed from the inhibited form of cathepsin B, and the activity of the active cathepsin B present in the sample is subsequently determined.