Purifying method for recombinant human granulocyte stimulating factor

Purifying method for recombinant human granulocyte stimulating factor

The invention relates to a purifying method for a recombinant human granulocyte stimulating factor. The method comprises the following steps of a. performing fermentation culture by selecting engineering bacteria as bacterial strains; b. separating and washing inclusion bodies of the bacterial strai...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: CHEN YUJUN, ZHAO HAIDAN, SONG CHENGJUN, NIU GUOLING, YU JUNQING, WANG WEIQUAN, TAO YONGBAO, LU GANG, PANG RUI, LI BANGDONG, YANG FENGDUO
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a purifying method for a recombinant human granulocyte stimulating factor. The method comprises the following steps of a. performing fermentation culture by selecting engineering bacteria as bacterial strains; b. separating and washing inclusion bodies of the bacterial strains obtained from the fermentation culture to obtain refined inclusion bodies; c. performing renaturation on the refined inclusion bodies to obtain a renaturation preoduct; d. performing anionic column chromatography treatment on the renaturation product to obtain an anionic column chromatography product; e. performing cation column chromatography treatment on the anionic column chromatography product to obtain a cation column chromatography product; and f. processing the cation column chromatography product by using a reverse phase packing column chromatography, and performing desalinizing treatment to obtain the recombinant human granulocyte colony-stimulating factor.