Typhoid fever salmonella and salmonella paratyphi fluorescent quantitative polymerase chain reaction (PCR) detection kit and application thereof

The invention belongs to the field of biotechnology and medical science, and provides a typhoid fever salmonella and salmonella paratyphi fluorescent quantitative polymerase chain reaction (PCR) detection kit. The detection kit comprises a first reaction solution, a second reaction solution and paraffin, wherein the first reaction solution comprises a reaction buffer solution, a primer pair and an ecprobe aiming at typhoid fever salmonella, a primer pair and an ecprobe aiming at the salmonella paratyphi. The sequence of the primer pair aiming at the typhoid fever is SEQ ID NO: 1 and SEQ ID NO: 2, the sequence of the ecprobe aiming at the typhoid fever is SEQ ID NO: 3, the sequence of the primer pair aiming at the salmonella paratyphi is SEQ ID NO: 3 and SEQ ID NO: 3, and the sequence of the ecprobe aiming at the salmonella paratyphi is SEQ ID NO: 6. The second reaction solution comprises deoxyribonucleic acid (DNA) polymerase O. The invention further provides an application of the detection kit in detection o