Step-by-step amplification culture method of bioleaching engineering bacteria in alpine region

The invention relates to a step-by-step amplification culture method of bioleaching engineering bacteria in an alpine region. The method comprises the following steps: carrying out steam heating of a bacterial liquid in a culture pool in a case that the outdoor temperature is -20-20DEG C, controlling the pressure of heating steam to make the temperature of the culture pool constantly between 20DEG C and 35DEG C, transferring to a culture pool having a large volume when the concentration of the bacterial liquid reaches 10 -10 /mL, and reducing the application of a culture medium agent step by step. The culture method allows the change ranges of the concentrations of N and P in the culture liquid to be very wide, the addition of extra nutritional liquids to be not needed, and onsite raffinade or tailing pond water to be adopted as a culture medium, and overcomes the disadvantages comprising large weather influences, slow culture speed, low efficiency, difficult condition control and the like during the culture