Construction method for linearized expression vector and simulated vector segments used for construction of linearized expression vector

The invention provides a construction method for a linearized expression vector and simulated vector segments used for the construction of the linearized expression vector. The construction method for the linearized expression vector comprises the following steps of designing and constructing a front simulated vector segment, a back simulated vector segment and a target gene segment; and connecting the front simulated vector segment, the back simulated vector segment and the target gene segment with ligase to obtain a linearized double-strand DNA expression vector. The invention further provides the simulated vector segments used for the construction of the linearized expression vector. By preparing simulated linearized vector segments through efficient and accurate connection reaction, a process of obtaining the linearized vector segments through the construction of complex and time-consuming ring-shaped plasmid vectors can be avoided; and simultaneously, and the generation of target gene terminals is not li