Immobilized method of creatine kinase

The invention provides an immobilized method of creatine kinase. The immobilized method uses chitosan as a raw material, a crosslinking chitosan solution is prepared firstly, a crosslinking chitosan microsphere is prepared, stem grafting lysine crosslinking chitosan resin is prepared then, and finally the stem grafting lysine crosslinking chitosan resin serves as a carrier to immobilize the creatine kinase. The immobilized method has the advantages that conventional production equipment can be used for producing phosphocreatine under a soft condition, a process is simple, operation is simple and convenient, cost of production is low, work efficiency is improved, and a productive process is favorable for environment protection. Stability of prepared immobilized creatine kinase on conditions such as heat, acid, alkali and an organic solvent is obviously improved.