Indirect ELISA (enzyme linked immunosorbent assay) method for detecting soybean antigenic protein serum antibody

The invention discloses an indirect ELISA (enzyme linked immunosorbent assay) method for detecting soybean antigenic protein serum antibody. The indirect ELISA method includes using purified soybean antigenic protein as antigenic peridium to detect soybean antigenic protein serum antibody. The soybean antigenic protein comprises glycinin and beta-conglycinin. The indirect ELISA method has the advantages that the method has high sensitivity and specificity to allergic diseases, which are caused by the soybean antigenic protein, of humans and animals, a basis is provided for diagnosis of the diseases, soybean protein source food allergy of animals in our country is convenient to overcome, effective technical means is provided for disease research of the diseases, and the quick and simple detecting method is provided for mastering prevention and morbidity of the soybean antigenic protein allergic diseases in humans and animals.