Method for detecting fungaltoxin by using photonic crystal microsphere flow type chip

The invention relates to a method for detecting fungaltoxin by using a photonic crystal microsphere flow type chip. According to the method, a photonic crystal microsphere is used as a fungaltoxin probe carrier; the surface of the microsphere is directly subjected to competitive immune, so that a plurality of fungaltoxins are detected; aflatoxin, Fumonisins and penicillium citrinum toxin are used as detection objects; a fluorescein isothiocyanate marked antibody competes with an antigen fixed on the surface of the microsphere to obtain own antigens; decoding is performed by a confocal fluorescence scanner and a reflection spectrum of a photonic crystal; and the three fungaltoxins can be detected at the same time. According to the method, the detection limit to the aflatoxin, the Fumonisins and the penicillium citrinum toxin is 0.001 ng/mL; the linear detection range is 0.001 to 10 ng/mL; only 10 mu L test sample is needed; and the whole analysis process is shorter than 3 hours.