Preparation method for pyrethroids pesticide degrading enzyme

The invention relates to the technical field of a preparation method for pesticide degrading enzyme, in particular to a preparation method for pyrethroids pesticide degrading enzyme. The preparation method for the pyrethroids pesticide degrading enzyme comprises the following steps of: 1, taking recombinant escherichia coli (E.coliBL21(DE3)/pET-28a-est825) as a production strain, and storing the production strain at the temperature of 86DEG C below zero by a glycerol tube; 2, carrying out seed culture; 3, carrying out fermentation culture; 4, carrying out feed supplement and fermentation culture; 5, carrying out lactose induction; and 6, preparing enzyme powder. According to the preparation method for the pyrethroids pesticide degrading enzyme, the recombinant escherichia coli is adopted to efficiently express the pyrethroids pesticide degrading enzyme, the yield of the finally obtained degrading enzyme powder is 25-40g/, the enzyme activity of the enzyme powder is 48000-60000U/g, the yield of the pyrethroids