High-density culture method of clostridium thermocellum

The invention belongs to the field of microbial fermentation engineering, and specifically relates to a high-density culture method of clostridium thermocellum. The method is characterized in that a GS-2 culture medium is used as an initial culture medium; the fermentation medium is optimized throug...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG HUAREN, ZHANG JINGTAO, ZHU XINSHU, CUI QIU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of microbial fermentation engineering, and specifically relates to a high-density culture method of clostridium thermocellum. The method is characterized in that a GS-2 culture medium is used as an initial culture medium; the fermentation medium is optimized through a multivariate statistical method; and cell concentration of clostridium thermocellum is rapidly and efficiently raised. The invention has the following advantage: the invention provides a fermentation medium for rapid acquisition of large cell concentration of clostridium thermocellum, thus laying the groundwork for subsequent production of cellulosome which has high conversion efficiency of cellulose to bioethanol. Bacterial density of clostridium thermocellum is raised by 6-9 times in comparison with that of the initial culture medium. OD600 under the bottling condition reaches 8.85, and OD600 in a fermentation cylinder reaches 12.7.