Haynaldia villosa 6VS chromosome specific molecular marker 6VS-UT and its application

Relating to the technical field of molecular biology and genetic breeding science, the invention discloses a molecular marker 6VS-UT specifically tracing Haynaldia villosa 6VS chromosome and its application. In the invention, primers can be designed according to a wheat EST (expressed sequence tag)...

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Bibliographische Detailangaben
Hauptverfasser: HUO JINJIN, LI JINGJING, ZHU SHANYING, HE HUAGANG, BIE TONGDE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:Relating to the technical field of molecular biology and genetic breeding science, the invention discloses a molecular marker 6VS-UT specifically tracing Haynaldia villosa 6VS chromosome and its application. In the invention, primers can be designed according to a wheat EST (expressed sequence tag) sequence BE445603 able to be electronically positioned on the short arm of chromosome 3 of brachypodium distachyon, corresponding DNA can be amplified and cloned from Haynaldia villosa and common wheat genomes, and based on sequencing, specific molecular marker primers can be further designed, with the concrete sequences shown as SEQIDNO.5 and SEQIDNO.6. When conducting PCR on the marker primers, a 301bp specific DNA band can be amplified, so that Haynaldia villosa 6VS chromosome and anti-wheat powdery mildew traits controlled thereby can be quickly and effectively traced. Thus, the Haynaldia villosa 6VS chromosome specific molecular marker 6VS-UT has a strong practical value in wheat anti-powdery mildew molecular