Method for shortening plant cell suspension culture period

Belonging to the field of biotechnologies, the invention relates to a method for shortening a plant cell suspension culture period. The method consists of: first, selecting forward fragments I and II from two conserved regions of gene p53 respectively, as well as corresponding reverse sequences I' and II'; constructing RNAi fragments of I-GU-II'-II-AG-I'; screening out the agrobacterium tumefaciens engineering bacterium inserted in a pCAMBIA2300 plant expression vector; then taking licorice for example, performing agrobacterium-mediated transformation to obtain a p53 transformed callus and its stably expressed suspension cell in order; then, carrying out nursing culture, and making use of the suspension cell to screen a single cell line callus characterized by fast growth speed and good effect, and a suspension cell line formed thereby; and finally, conducting culture process optimization to obtain the optimum fermentation process parameters of suspension cell growth and product accumulation. The method provi