Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof

The invention relates to a duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof, which belong to the field of bio-pharmaceuticals. The preparation method of the DTMUV disease immunotherapy preparation comprises the following steps of: culturing seed viruses for...

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Hauptverfasser: DONG JIAWEN, HUANG ZHISONG, CHENG HUAILING, QIU ZHIXIN, HU QILIN, LI LINLIN, WEI SUHONG, YUAN JIANFENG, WEI PINGHUA, SUN MINHUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof, which belong to the field of bio-pharmaceuticals. The preparation method of the DTMUV disease immunotherapy preparation comprises the following steps of: culturing seed viruses for producing a tembusu virus strain on a large scale, and harvesting a virus liquid; deactivating the collected virus liquid, concentrating, adding the deactivated and concentrated virus liquid into an adjuvant, and emulsifying to obtain an immune antigen; immunizing laying ducks by using the immune antigen, randomly collecting blood and separating serum after last immunization, and measuring the valence of an anti-DTMUV antigen through an Agar Gel Precipiti (AGP) test till the valance of the antigen is qualified; and collecting eggs laid by an immunized qualified duck group, and preparing into a high-immunity egg yolk antibody with the conventional method. The DTMUV disease immunotherapy preparation contains a DTMUV