Method for inducing garlic test tube bulb

The invention discloses a new method for inducing a garlic test tube bulb. The method mainly comprises the following steps of: performing low-temperature treatment of the garlic tender bud at 4 DEG C to induce atmogenic bulbil; rejuvenating atmogenic bulbil on an MS+2.5mg KT+0.5mg NAA culture medium...

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Bibliographische Detailangaben
Hauptverfasser: LI ZHUANCHUN, PENG AIFANG, WANG ZHENYING, FAN BAOLI, CHEN HONG, LIU XIAOYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a new method for inducing a garlic test tube bulb. The method mainly comprises the following steps of: performing low-temperature treatment of the garlic tender bud at 4 DEG C to induce atmogenic bulbil; rejuvenating atmogenic bulbil on an MS+2.5mg KT+0.5mg NAA culture medium; and inducing the test tube bulb on an MS+60g/L sucrose culture medium without hormone. The method can be used for improving the propagation efficiency of garlic rapid propagation by using atmogenic bulbil as seeds, and reducing the cost. By virtue of the method disclosed by the invention, the atmogenic bulbil induction rate can reach 100% (each bud can induce out atmogenic bulbil), each bud can induce out 25 atmogenic bulbils, all of the atmogenic bulbils can be induced into test tube bulbs, and the test tube bulb induction rate reaches 100%. Without cell dedifferentiation and redifferentiation in the whole induction process, the genetic mutation probability is reduced; and the method disclosed by the invention p