Colloidal gold test strip for detecting residue of sulfonamides, usage method and application thereof

The invention discloses a colloidal gold test strip, which comprises an absorption pad, a nitrocellulose membrane, a gold-labeled pad and a sample pad that are sequentially laminated and pasted on a plastic lining board. The cellulose nitrate membrane is provided with a detection line and a quality control line; the gold-labeled pad is coated with colloidal gold-labeled monoclonal antibodies capable of identifying sulfonamides; the detection line is coated with a sulfonamide coating antigen; and the quality control line is coated with a goat or rabbit anti-rat IgG. The invention also discloses a usage method of the colloidal gold test strip and application of the colloidal gold test strip to detection of residues of sulfonamides. The colloidal gold test strip provided by the invention can be used for detecting residues of sulfonamides in tissue samples, such as milk, pork, chicken, beef, lamb, fish, shrimp, pork liver, chicken liver, egg and honey, etc.; and the colloidal gold test strip provided by the inven