Method for extracting DNA (deoxyribonucleic acid) by alkaline cracking

The invention provides a method for extracting DNA (deoxyribonucleic acid) by alkaline cracking. The method comprises the following steps of: 1, putting testing material into NaOH (sodium hydroxide) solution of which the concentration is 0.05-0.6M; 2, carrying out incubation on solution, wherein the incubation temperature is 90-99 DEG C and the incubation time is 5-10 minutes; 3, oscillating the incubated solution in an oscillator to oscillate; 4, adding Tris-HCl (tris(hydroxymethyl)aminomethane hydrochloride) solution of which the concentration is 0.01-0.1M and the pH value is 5-8 and carrying out centrifugal oscillation, and 5, freezing and storing extract at the temperature of 20 DEG C. Compared with the prior art, the requirements of existing various conventional forensic biological test materials can be met by changing parameters such as the concentration, the pH value, the incubation time, the incubation temperature and the like of reagent, and automatic DNA extraction work can be carried out at the sam