Construction method for prokaryotic secretory expression vector of protein transduction domain-Apoptin (PTD-Apoptin) fusion protein and application of prokaryotic secretory expression vector

The invention relates to a construction method for a prokaryotic secretory expression vector of a protein transduction domain-Apoptin (PTD-Apoptin) fusion protein and the application of the prokaryotic secretory expression vector. The construction method comprises the following steps of: connecting a PTD-Apoptin sequence onto a pET22b(+) secretory expression vector to construct the prokaryotic secretory expression vector pET22b(+)-PTD-Apoptin; secreting an expressed interest protein into the periplasmic space of escherichia coli through isopropyl thiogalactoside (IPTG) induction; extracting the protein of the periplasmic space by an osmose process; performing ultrasonication on remaining cellular components; then, separating supernatant and depositing; and performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Co-incubation is performed on the PTD-Apoptin fusion protein in the periplasmic space and a gastric cancer cell-823, and the inhibitory actionof the fusion protein on