Detection method for enzyme-linked immune adsorption of 2-naphthylamine in food

The invention relates to a detection method for enzyme-linked immune adsorption of 2-naphthylamine in food and belongs to the field of immunology detection analysis. The detection method comprises the following steps: enveloping the same amount of antigens in holes of an ELISA plate; adding to-be-tested 2-naphthylamine-containing food sample and 2-naphthylamine polyclonal antibody, and then causing the 2-naphthylamine in the to-be-tested sample and solid-phase envelope antigen to fight for limited first antibodies and combine with the antibody, wherein few antibodies are combined with the solid-phase envelope antigen when the concentration of the 2-naphthylamine in the to-be-tested sample is high; after achieving reaction balance, pouring out supernatant; washing with washing liquor; remaining the antibodies combined with the solid-phase envelope antigen in the holes of the ELISA plate and reacting with the added ELIAS secondary antibody; and if the combining volume of the ELIAS secondary antibody with the fi