Ultrafast detection method for nucleic acid

The invention relates to an ultrafast detection method for nucleic acid. The method is characterized by extracting nucleic acid, performing an amplification in a special buffer solution and a disk type reactor under the catalysis of an enzyme using a fluorescent-labeled primer and detecting quantita...

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Bibliographische Detailangaben
Hauptverfasser:	KANG FEI, WANG BAOJUN, TAO JIANGUO, XIAO LEYI, CAI YINGYING, FENG XIAOCHENG, GONG HONGLING, FENG CHANGFANG, XIE KAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an ultrafast detection method for nucleic acid. The method is characterized by extracting nucleic acid, performing an amplification in a special buffer solution and a disk type reactor under the catalysis of an enzyme using a fluorescent-labeled primer and detecting quantitatively the content of nucleic acid templates in samples in real time. The special buffer solution and the disk type reactor enable the reaction time to be shortened greatly compared with that of conventional fluorescent quantitative detection method for the nucleic acid, to be shortened from an original time of over one hour to a present time of 5-10 minutes, so that a fast detection for pathogen infections is really realized. The method has the advantages of short reaction time, simple process and high specificity, is particularly suitable for detecting emerging acute infectious diseases, and has high popularization value.