In-vitro culture method for tender stem segments of cherry rootstock
The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleani...
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| creator | TANG GUIJUN JIANG JIANPING LU JINMING HU HAIFENG WANG SHUIYAN ZHU TIANHUA |
| description | The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to s |
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The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to s</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED ; FISHING ; FORESTRY ; HORTICULTURE ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING ; WATERING</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120912&DB=EPODOC&CC=CN&NR=102657098A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120912&DB=EPODOC&CC=CN&NR=102657098A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TANG GUIJUN</creatorcontrib><creatorcontrib>JIANG JIANPING</creatorcontrib><creatorcontrib>LU JINMING</creatorcontrib><creatorcontrib>HU HAIFENG</creatorcontrib><creatorcontrib>WANG SHUIYAN</creatorcontrib><creatorcontrib>ZHU TIANHUA</creatorcontrib><title>In-vitro culture method for tender stem segments of cherry rootstock</title><description>The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to s</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HORTICULTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><subject>WATERING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0OgkAQBtBtLIx6h_EAJKjxrzSo0cbKnpDlWyGwO2RmMPH2Nh7A6jVv6s73lL1bEyY_9jYKKMIarimwkCHVEFJDJMUrIpkSB_INRD4kzKbGvpu7Sah6xeLnzC2vl2dxyzBwCR0qjwQri8cqX--2-_x4OG3-OV_R3zMm</recordid><startdate>20120912</startdate><enddate>20120912</enddate><creator>TANG GUIJUN</creator><creator>JIANG JIANPING</creator><creator>LU JINMING</creator><creator>HU HAIFENG</creator><creator>WANG SHUIYAN</creator><creator>ZHU TIANHUA</creator><scope>EVB</scope></search><sort><creationdate>20120912</creationdate><title>In-vitro culture method for tender stem segments of cherry rootstock</title><author>TANG GUIJUN ; JIANG JIANPING ; LU JINMING ; HU HAIFENG ; WANG SHUIYAN ; ZHU TIANHUA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN102657098A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2012</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HORTICULTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><topic>WATERING</topic><toplevel>online_resources</toplevel><creatorcontrib>TANG GUIJUN</creatorcontrib><creatorcontrib>JIANG JIANPING</creatorcontrib><creatorcontrib>LU JINMING</creatorcontrib><creatorcontrib>HU HAIFENG</creatorcontrib><creatorcontrib>WANG SHUIYAN</creatorcontrib><creatorcontrib>ZHU TIANHUA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TANG GUIJUN</au><au>JIANG JIANPING</au><au>LU JINMING</au><au>HU HAIFENG</au><au>WANG SHUIYAN</au><au>ZHU TIANHUA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>In-vitro culture method for tender stem segments of cherry rootstock</title><date>2012-09-12</date><risdate>2012</risdate><abstract>The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to s</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING WATERING |
| title | In-vitro culture method for tender stem segments of cherry rootstock |
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