In-vitro culture method for tender stem segments of cherry rootstock

The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to s