Preparation and detection method of textile fiber digitized slice and microscopic examination simulation method

The invention discloses a preparation and a detection method of textile fiber digitized slice and a microscopic examination simulation method. The digitized slice contains image information of a fiber sample loaded by a glass slice. The preparation method of the textile fiber digitized slice includes steps of: an image acquisition step: observing multiple sampling areas of the glass slice loaded with fiber sample by using a microscope; acquiring multiple direct observation images of multiple focus layers in each corresponding sampling areas, by using an image collecting device; and a digital slice generation step: storing the digitized slice into a computer readable storage medium, wherein the digitized slice includes all acquired direct observation images. Each image of the digitized slice prepared by the preparation method of the invention is a real digitized direct observation image, and contains complete original information, can be used to reproduce the detection process; therefore, it is especially suit