Specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for transgenic corn 59122 strain
The invention belongs to a biological technology, and relates to the quantitative analyzing method of genes, in particular to a specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for a transgenic corn 59122 strain. In the method, a PCR reaction system is prepared by adop...
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| creator | SONG JUN ZHANG FULI YIN QUAN CHANG LIJUAN LIU WENJUAN WANG DONG LEI SHAORONG GUO LINGAN LIU YONG |
| description | The invention belongs to a biological technology, and relates to the quantitative analyzing method of genes, in particular to a specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for a transgenic corn 59122 strain. In the method, a PCR reaction system is prepared by adopting a designed specific upstream primer 59122event-F, a downstream primer 59122event-R, a fluorescent probe 59122event-P, a DNA diluent of the 59122 strain, TaqmanMastermix and water for performing quantitative PCR detection. In the method, a Taqman quantitative PCR detection technology with high amplification efficiency and high accuracy is mainly established; and the method is suitable for supervision and inspection of domestic agricultural transgenic organisms and products, inspection of entry-exit port transgenic organisms and products, and biological component detection of enterprise internal import raw materials containing transgenic 59122 strains. |
| format | Patent |
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In the method, a PCR reaction system is prepared by adopting a designed specific upstream primer 59122event-F, a downstream primer 59122event-R, a fluorescent probe 59122event-P, a DNA diluent of the 59122 strain, TaqmanMastermix and water for performing quantitative PCR detection. In the method, a Taqman quantitative PCR detection technology with high amplification efficiency and high accuracy is mainly established; and the method is suitable for supervision and inspection of domestic agricultural transgenic organisms and products, inspection of entry-exit port transgenic organisms and products, and biological component detection of enterprise internal import raw materials containing transgenic 59122 strains.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120801&DB=EPODOC&CC=CN&NR=102618657A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120801&DB=EPODOC&CC=CN&NR=102618657A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SONG JUN</creatorcontrib><creatorcontrib>ZHANG FULI</creatorcontrib><creatorcontrib>YIN QUAN</creatorcontrib><creatorcontrib>CHANG LIJUAN</creatorcontrib><creatorcontrib>LIU WENJUAN</creatorcontrib><creatorcontrib>WANG DONG</creatorcontrib><creatorcontrib>LEI SHAORONG</creatorcontrib><creatorcontrib>GUO LINGAN</creatorcontrib><creatorcontrib>LIU YONG</creatorcontrib><title>Specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for transgenic corn 59122 strain</title><description>The invention belongs to a biological technology, and relates to the quantitative analyzing method of genes, in particular to a specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for a transgenic corn 59122 strain. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for transgenic corn 59122 strain |
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