Specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for transgenic corn 59122 strain

The invention belongs to a biological technology, and relates to the quantitative analyzing method of genes, in particular to a specific quantitative PCR (Polymerase Chain Reaction) accurate detection method for a transgenic corn 59122 strain. In the method, a PCR reaction system is prepared by adopting a designed specific upstream primer 59122event-F, a downstream primer 59122event-R, a fluorescent probe 59122event-P, a DNA diluent of the 59122 strain, TaqmanMastermix and water for performing quantitative PCR detection. In the method, a Taqman quantitative PCR detection technology with high amplification efficiency and high accuracy is mainly established; and the method is suitable for supervision and inspection of domestic agricultural transgenic organisms and products, inspection of entry-exit port transgenic organisms and products, and biological component detection of enterprise internal import raw materials containing transgenic 59122 strains.