Construction method of Small RNA-Seq library for screening early diagnosis targets of tumor
The invention relates to a construction method of a Small RNA-Seq library for screening early diagnosis targets of a tumor, and in particular relates to a method for constructing a Small RNA-Seq library for next-generation sequencing aiming at less blood samples of a single individual. According to...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a construction method of a Small RNA-Seq library for screening early diagnosis targets of a tumor, and in particular relates to a method for constructing a Small RNA-Seq library for next-generation sequencing aiming at less blood samples of a single individual. According to the method, targeted fragments are recovered through plasma separation, total RNA extraction, RT-PCR (reverse transcription-polymerase chain reaction) and PAGE (PolyAcrylamide Gel Electrophoresis), so that the Small RNA-Seq library suitable for target screening and target diagnosis can be constructed. |
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