Construction method of Small RNA-Seq library for screening early diagnosis targets of tumor

The invention relates to a construction method of a Small RNA-Seq library for screening early diagnosis targets of a tumor, and in particular relates to a method for constructing a Small RNA-Seq library for next-generation sequencing aiming at less blood samples of a single individual. According to...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LIU ZHENZHEN, DONG ENCHENG, WU ZHONGYI, LV XUEMEI, LI CHUNYAN
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to a construction method of a Small RNA-Seq library for screening early diagnosis targets of a tumor, and in particular relates to a method for constructing a Small RNA-Seq library for next-generation sequencing aiming at less blood samples of a single individual. According to the method, targeted fragments are recovered through plasma separation, total RNA extraction, RT-PCR (reverse transcription-polymerase chain reaction) and PAGE (PolyAcrylamide Gel Electrophoresis), so that the Small RNA-Seq library suitable for target screening and target diagnosis can be constructed.