Method for extracting Phanerochaete chrysosporium exocytosis protein under heavy metal induction
The invention discloses a method for extracting Phanerochaete chrysosporium exocytosis protein under heavy metal induction. The method comprises the following steps of: firstly carrying out liquid-state culturing on Phanerochaete chrysosporium, adding cadmium ion solution to culture, and filtering t...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for extracting Phanerochaete chrysosporium exocytosis protein under heavy metal induction. The method comprises the following steps of: firstly carrying out liquid-state culturing on Phanerochaete chrysosporium, adding cadmium ion solution to culture, and filtering to obtain exocytosis fluid after heavy metal induction; adding high-percentage-concentration trichloroacetic acid solution to the exocytosis fluid, precipitating overnight, abandoning supernatant liquid after centrifugation, and collecting precipitate, wherein the addition amount of the high-percentage-concentration trichloroacetic acid solution is 1/9-1/10 the volume of the exocytosis fluid; and finally washing precipitate by using acetone solution, carrying out centrifugation, and abandoning the supernatant to obtain exocytosis protein. The method disclosed by the invention has the advantages of simpleness, efficiency and capabilities of precipitating a large amount of protein and reducing the volume of liquid to |
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