Nucleic acid marker for gel electrophoresis as well as preparation method and application thereof

The invention provides a nucleic acid marker for polyacrylamide gel electrophoresis, a preparation method of the nucleic acid marker for gel electrophoresis, and a method for using the nucleic acid marker for gel electrophoresis to detect nucleic acid of gel electrophoresis. The nucleic acid marker...

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Bibliographische Detailangaben
Hauptverfasser:	GAO FENGHOU, SHI JING, JIANG BIN, GUO YUEHUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a nucleic acid marker for polyacrylamide gel electrophoresis, a preparation method of the nucleic acid marker for gel electrophoresis, and a method for using the nucleic acid marker for gel electrophoresis to detect nucleic acid of gel electrophoresis. The nucleic acid marker for gel electrophoresis comprises a single-stranded DNA (deoxyribonucleic acid) and further comprises a DNA- DNA double strand and/or DNA- RNA (Ribonucleic Acid) hybridized double strand; wherein, base numbers of two single-stranded DNAs in the DNA-DNA double strand are the same and the bases are complementary; and base number of a single-stranded DNA and that of a single-stranded RNA in the DNA- RNA double strand are the same, and the bases are complementary. The nucleic acid marker can be used for molecule comparison of non-denaturing PAGE (polyacrylamide gel electrophoresis) after solution hybridization and is particularly suitable for detection of 200bp nucleic acid.