Kit and method for detecting porcine proliferative enteropathy (PPE) pathogenic bacteria Lawsonia Intracellularis

The invention belongs to the field of pathogenic inspection and quarantine of infectious disease of livestock, and particularly relates to a kit and a method for detecting Lawsonia Intracellularis. In order to realize the purpose of the invention, the technical scheme adopted by the invention is to provide a kit for detecting porcine proliferative enteropathy (PPE) pathogenic bacteria Lawsonia Intracellularis, wherein the kit comprises a primer pair for amplifying DNA (deoxyribonucleic acid) segments of a sample to be detected in the DAN of the extracted sample. According to the invention, through sufficient test screening and optimization, and original creation of a primer probe, reaction conditions, positive control and optimal matching conditions, the method capable of quickly and reliably carrying out PCR (polymerase chain reaction) detection on PPE is established. According to the invention, quantification of a DNA template is realized; the kit and the method have the characteristics of high sensitivity,