Fluorescence quantitative PCR (polymerase chain reaction) detection kit for salmonella and detection method thereof

The invention relates to a fluorescence quantitative PCR (polymerase chain reaction) detection kit for salmonella, comprising a salmonella PCR reaction liquid, a Taq enzyme, a positive control and a negative control. The sequences of the specific primers of the fluorescence quantitative PCR detectio...

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Bibliographische Detailangaben
Hauptverfasser:	BAI PEISHENG, WEN YUNJIE, WANG XIAODAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a fluorescence quantitative PCR (polymerase chain reaction) detection kit for salmonella, comprising a salmonella PCR reaction liquid, a Taq enzyme, a positive control and a negative control. The sequences of the specific primers of the fluorescence quantitative PCR detection kit for salmonella are as follows: the upstream primer is 5'-TGCGCCTGCCGTTATCAC-3'; the downstream primer is 5'-ATTTGAGCTGTTGGTTCAGTAACTC-3'; and the fluorescence probe sequence is 5'-CCAACGCCGCAGATATTTGATCGCC-3'. The detection for salmonella in a clinical excrement by using the kit disclosed by the invention has great specificity, sensitivity and stability, the whole detection process can be finished in 2-3 hours, and salmonella in foods or other cultures also can be rapidly and accurately detected.