Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode

The invention relates to a Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode and a manufacturing method and an application method thereof. The manufacturing method for the electrode comprises the following steps of: polishing a stainless steel acupuncture needle on an animal skin; repeatedly washing by secondary distilled water and ultrasonically processing for 5-10min in an ultrasonic washer with power of 100-150W; placing in a phosphate buffered solution at pH7 and activating for 10-15 circles through cyclic sweep voltammetry; dissolving 49-60mg of chitosan in 80-120ml of 0.05mol/L of acetic acid solution to obtain 0.4-0.6% of chitosan solution; and dissolving the manufactured Pt-Ag alloy nanoparticle in 0.5ml of 0.4-0.6% of chitosan solution, ultrasonically dispersing, dispensing 40-60 mu l of the ultrasonically dispersed solution onto an Au electrode, and drying at room temperature. The Pt-Ag alloy nanoparticle electrode has good catalytic activity, excellent electrocatalytic activity for gluc