Human mitochondrial DNA (deoxyribonucleic acid) purification method

The invention relates to a human mitochondrial DNA purification method. The method utilizes the joint action of restriction endonucleases (Bg1 II and Dra III), ribonuclease A and exonuclease III to degrade nuclear DNA and RNA (Ribonucleic acid) residues in genome DNA or mitochondrial DNA crude extra...

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Bibliographische Detailangaben
Hauptverfasser:	LV JIANXIN, FANG FANG, ZHENG JING, ZHENG BINJIAO, ZHANG TING, GONG SHASHA, GUAN MINXIN, LIANG MIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a human mitochondrial DNA purification method. The method utilizes the joint action of restriction endonucleases (Bg1 II and Dra III), ribonuclease A and exonuclease III to degrade nuclear DNA and RNA (Ribonucleic acid) residues in genome DNA or mitochondrial DNA crude extract, so that high-purity mitochondrial DNA can be obtained. The method is widely applicable to the purification of human mitochondrial DNA samples, the purity is high, and the method is easy to operate, takes short time, and can meet the requirement of each technology in the mitochondria research field on the high purity of mitochondrial DNA.