Transgenic corn NK603 strain specific quantitative PCR (Polymerase Chain Reaction) accurate detection method

The invention belongs to a biotechnology, and relates to a quantitative analysis method for genes, in particular to a transgenic corn NK603 strain specific quantitative PCR (Polymerase Chain Reaction) accurate detection method. In the invention, designed specific upstream primer Event 603-F, downstr...

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Bibliographische Detailangaben
Hauptverfasser:	SONG JUN, ZHANG FULI, YIN QUAN, CHANG LIJUAN, LIU WENJUAN, LEI SHAORONG, WANG DONG, GUO LINGAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to a biotechnology, and relates to a quantitative analysis method for genes, in particular to a transgenic corn NK603 strain specific quantitative PCR (Polymerase Chain Reaction) accurate detection method. In the invention, designed specific upstream primer Event 603-F, downstream primer Event 603-R, a fluorescent probe Event 603-P, DNA (Deoxyribonucleic Acid) diluent of NK603 strain, TaqmanMastermix and water are prepared into a PCR reaction system; and quantitative PCR detection is performed. In the invention, a Taqman quantitative PCR detection technology of high amplification efficiency and high accuracy is mainly established; and the method is suitable for supervision and inspection of domestic agricultural transgenic organisms and products, inspection of entry and exit port transgenic organisms and products and detection of transgenic NK603 strain biological ingredient included in imported raw materials in enterprises.