Specific quantitative polymerase chain reaction (PCR) detection method for transgenic corn MON863 strain

The invention belongs to a biological technology, and relates to a quantitative analytical method for genes, in particular to a specific quantitative polymerase chain reaction (PCR) detection method for a transgenic corn MON863 strain. The method comprises the following steps of: preparing a PCR reaction system from a specific forward primer Event863-F, a specific reverse primer Event863-R, a fluorescent probe Event863-P, deoxyribonucleic acid (DNA) diluent of the MON863 strain, TaqmanMastermix and water; and performing quantitative PCR detection. In the method, a Taqman quantitative PCR detection technology with high amplification efficiency and accuracy is established, and the method is suitable for the monitoring and inspection of transgenic organisms and transgenic products of domestic agriculture, the inspection of the transgenic organisms and the transgenic products of import and export ports and the detection of ingredients of organisms containing the transgenic MON863 strain in import raw materials of