Method for inducing callus of lycoris radiate by using flower stalks
The invention discloses a method for callus of lycoris radiate by using flower stalks, comprising the following steps: taking the flower stalks of the lycoris radiate after being sterilized; inoculating in induction culture medium, cultivating for 28-32 d in sunshine, then moving to a dark environme...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for callus of lycoris radiate by using flower stalks, comprising the following steps: taking the flower stalks of the lycoris radiate after being sterilized; inoculating in induction culture medium, cultivating for 28-32 d in sunshine, then moving to a dark environment, and cultivating for 88-92 d, wherein the induction culture medium comprises 2.0-2.5 mg/L of 2,4-D and 1.5-2.5 mg/LBA of MS culture medium, and the MS culture medium comprises sugarcane of which concentration is 30-60 g/L and agar of which concentration is 5-8 g/L and pH is 5.6-5.8. The invention adopts the flower stalks of the lycoris radiate as the exophyte, produces the callus of the lycoris radiate through cultivating in the induction culture medium, cannot be at the cost of consuming the bulbs, and effectively protects the genetic resources of the rare lycoris radiate which has good quality. |
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