Digestive juice for separating mouse fat stem cells

The invention discloses digestive juice for separating mouse fat stem cells. The digestive juice comprises 10% of fetal calf serum, 0.09% of collagenase I and the balance of DMEM/F12 (dulbecco's modified eagle medium/nutrient mixture F-12). The digestive juice is simple in components, convenien...

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Bibliographische Detailangaben
Hauptverfasser:	ZHOU NARU, GUI TAO, WU RONGHUA, ZHANG YUNHAI, WEI CHAO, CHEN JIANWEN, LI YUNSHENG, TAO JIA, ZHANG YU, LIU XING, ZHANG XIAORONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses digestive juice for separating mouse fat stem cells. The digestive juice comprises 10% of fetal calf serum, 0.09% of collagenase I and the balance of DMEM/F12 (dulbecco's modified eagle medium/nutrient mixture F-12). The digestive juice is simple in components, convenient in source and low in cost, and can effectively separate the fat stem cells from fat tissues of a mouse, well maintain the activity of the cells and lay a foundation for late-stage culture of the cells and further scientific research.