Simple method suitable for pathogenic water mold preservation
The invention provides a simple method suitable for pathogenic water mold preservation. The method comprises the following steps of: inoculating water mold to a potato dextrose agar culture medium plate on which sterile rape seeds are put, and performing thermostatic culture at the temperature of 25...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a simple method suitable for pathogenic water mold preservation. The method comprises the following steps of: inoculating water mold to a potato dextrose agar culture medium plate on which sterile rape seeds are put, and performing thermostatic culture at the temperature of 25 DEG C till hyphae are grown on the rape seeds; and putting the rape seeds grown with the hyphae into a sterile plastic centrifugal tube with a stopper, sucking sterile distilled water and liquid Salouraud culture medium into the sterile plastic centrifugal tube with the stopper by using a glass liquid transfer tube, and preserving the sterile plastic centrifugal tube with the stopper in a thermostatic refrigerator of 4 DEG C. Compared with a subculture preservation method, the method has the advantages that: the preservation period is longer, and the effective preservation time is at least 12 months; compared with a freeze-drying preservation method, the method has the advantages that: the operation is simpler and |
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