Reagent for detecting enterohemorrhagic escherichia coli by using cross primer nucleic acid isothermal amplification technology, amplification method and detection method for enterohemorrhagic escherichia coli
The invention discloses a reagent for detecting enterohemorrhagic escherichia coli by using a cross primer nucleic acid isothermal amplification technology, an amplification method and a detection method for enterohemorrhagic escherichia coli. Five primer group sequences are designed, namely 5'...
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| creator | WANG XIN GAO QIUPING WU TINGYING ZHANG XIA LIU ZHIYONG YOU QIMIN CHAI HONGSEN XU GAOLIAN WANG HONGYING ZUO FENG YANG CHUNJIANG CUI JINGBAI ZHAN XIJING QI JUN LIU YIN LIU QIJUN LIU ZHENYU |
| description | The invention discloses a reagent for detecting enterohemorrhagic escherichia coli by using a cross primer nucleic acid isothermal amplification technology, an amplification method and a detection method for enterohemorrhagic escherichia coli. Five primer group sequences are designed, namely 5'CAGTCGTACGGGGATGCAG, 5'TCAGCAGTCATTACATAAG, 5'GCTCTTGCCACAGACTGCGTC, 5'AGGTTCCGCTATGCGACAT and 5'GCTCTTGCCACAGACTGCGTCAGTTTCGCCATTCGTTGACTACTT. Aiming at the enterohemorrhagic escherichia coli O157:H7 bacteria, the defects in the prior art are overcome by the reagent, and a method for detecting the enterohemorrhagic escherichia coli quickly at low cost by using the cross primer nucleic acid isothermal amplification technology is provided. |
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Five primer group sequences are designed, namely 5'CAGTCGTACGGGGATGCAG, 5'TCAGCAGTCATTACATAAG, 5'GCTCTTGCCACAGACTGCGTC, 5'AGGTTCCGCTATGCGACAT and 5'GCTCTTGCCACAGACTGCGTCAGTTTCGCCATTCGTTGACTACTT. Aiming at the enterohemorrhagic escherichia coli O157:H7 bacteria, the defects in the prior art are overcome by the reagent, and a method for detecting the enterohemorrhagic escherichia coli quickly at low cost by using the cross primer nucleic acid isothermal amplification technology is provided.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120229&DB=EPODOC&CC=CN&NR=102363816A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120229&DB=EPODOC&CC=CN&NR=102363816A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG XIN</creatorcontrib><creatorcontrib>GAO QIUPING</creatorcontrib><creatorcontrib>WU TINGYING</creatorcontrib><creatorcontrib>ZHANG XIA</creatorcontrib><creatorcontrib>LIU ZHIYONG</creatorcontrib><creatorcontrib>YOU QIMIN</creatorcontrib><creatorcontrib>CHAI HONGSEN</creatorcontrib><creatorcontrib>XU GAOLIAN</creatorcontrib><creatorcontrib>WANG HONGYING</creatorcontrib><creatorcontrib>ZUO FENG</creatorcontrib><creatorcontrib>YANG CHUNJIANG</creatorcontrib><creatorcontrib>CUI JINGBAI</creatorcontrib><creatorcontrib>ZHAN XIJING</creatorcontrib><creatorcontrib>QI JUN</creatorcontrib><creatorcontrib>LIU YIN</creatorcontrib><creatorcontrib>LIU QIJUN</creatorcontrib><creatorcontrib>LIU ZHENYU</creatorcontrib><title>Reagent for detecting enterohemorrhagic escherichia coli by using cross primer nucleic acid isothermal amplification technology, amplification method and detection method for enterohemorrhagic escherichia coli</title><description>The invention discloses a reagent for detecting enterohemorrhagic escherichia coli by using a cross primer nucleic acid isothermal amplification technology, an amplification method and a detection method for enterohemorrhagic escherichia coli. Five primer group sequences are designed, namely 5'CAGTCGTACGGGGATGCAG, 5'TCAGCAGTCATTACATAAG, 5'GCTCTTGCCACAGACTGCGTC, 5'AGGTTCCGCTATGCGACAT and 5'GCTCTTGCCACAGACTGCGTCAGTTTCGCCATTCGTTGACTACTT. Aiming at the enterohemorrhagic escherichia coli O157:H7 bacteria, the defects in the prior art are overcome by the reagent, and a method for detecting the enterohemorrhagic escherichia coli quickly at low cost by using the cross primer nucleic acid isothermal amplification technology is provided.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjEKAkEMRbexEPUOsVdwXRBbEcXKQuyXmM3OBGYmy8xYeExv5AqKYKNV4Od9_hsW9xOj4ZCh1QgNZ6YswUCfcFTLXmO0aISAE1mOQlYQSJ3A5QbX9GQpakrQRfEcIVzJcY8jSQOSNPcljw7Qd05aIcyiAfoZG9Spuc2-Pp6z1QYwNG-bT_hU_C02LgYtusST1x0V0_3uvD3MudOaU4fEgXO9PZaLZbWq1uVqU_3DPABme2mL</recordid><startdate>20120229</startdate><enddate>20120229</enddate><creator>WANG XIN</creator><creator>GAO QIUPING</creator><creator>WU TINGYING</creator><creator>ZHANG XIA</creator><creator>LIU ZHIYONG</creator><creator>YOU QIMIN</creator><creator>CHAI HONGSEN</creator><creator>XU GAOLIAN</creator><creator>WANG HONGYING</creator><creator>ZUO FENG</creator><creator>YANG CHUNJIANG</creator><creator>CUI JINGBAI</creator><creator>ZHAN XIJING</creator><creator>QI JUN</creator><creator>LIU YIN</creator><creator>LIU QIJUN</creator><creator>LIU ZHENYU</creator><scope>EVB</scope></search><sort><creationdate>20120229</creationdate><title>Reagent for detecting enterohemorrhagic escherichia coli by using cross primer nucleic acid isothermal amplification technology, amplification method and detection method for enterohemorrhagic escherichia coli</title><author>WANG XIN ; 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Five primer group sequences are designed, namely 5'CAGTCGTACGGGGATGCAG, 5'TCAGCAGTCATTACATAAG, 5'GCTCTTGCCACAGACTGCGTC, 5'AGGTTCCGCTATGCGACAT and 5'GCTCTTGCCACAGACTGCGTCAGTTTCGCCATTCGTTGACTACTT. Aiming at the enterohemorrhagic escherichia coli O157:H7 bacteria, the defects in the prior art are overcome by the reagent, and a method for detecting the enterohemorrhagic escherichia coli quickly at low cost by using the cross primer nucleic acid isothermal amplification technology is provided.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Reagent for detecting enterohemorrhagic escherichia coli by using cross primer nucleic acid isothermal amplification technology, amplification method and detection method for enterohemorrhagic escherichia coli |
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