Purification method of marine bivalve shellfish blood cell

The invention relates to a purification method of a marine bivalve shellfish blood cell, which comprises the steps that: (1) fresh marine bivalve shellfish blood is mixed with an anticoagulant to obtain mixed liquid; (2) the mixed liquid is filtered and naturally settled, and then a surface is remov...

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Bibliographische Detailangaben
Hauptverfasser: YING CHENGQI, YAO ZONGLI, ZHOU KAI, LAI QIFANG, LI ZINIU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a purification method of a marine bivalve shellfish blood cell, which comprises the steps that: (1) fresh marine bivalve shellfish blood is mixed with an anticoagulant to obtain mixed liquid; (2) the mixed liquid is filtered and naturally settled, and then a surface is removed; centrifugation is carried out, and supernatant is removed; and finally the liquid is washed by a phosphate buffer solution (PBS) for twice, and the blood cell is obtained after being suspended. The purification method of the marine bivalve shellfish blood cell is simple and feasible without adding any instrument and equipment, improves the prescription of the anticoagulant, purifies the blood, solves the problem that the blood cell has a plurality of impurities in a shellfish blood cell research process, and provides practical and effective technical means for the research on shellfish blood cells.