Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method

The invention relates to the technical field of plant tissue culture, and particularly relates to a culture medium composition for rapid rooting of blueberry tissue culture seedlings outside a test tube and a preparation method. The composition comprises: a basal culture medium of WPM culture medium, wherein 20 g of white sugar is added into each liter of WPM culture medium; a rooting culture solution, wherein a culture medium diluent is obtained by diluting the basal culture medium by 6 times, and 25 mg of auxin is added into each liter of the culture medium diluent; a culture medium supporting substance of perlite, wherein the ratio of perlite and the rooting culture solution is 375 g/L. The culture medium composition for rapid rooting of blueberry tissue culture seedlings outside a test tube and the preparation method of the invention solves the problems of low rooting rate of blueberry tissue culture seedlings outside bottles (outside test tubes), unstable rooting condition, and long rooting time.