Kit for detecting beta-lactamase in raw milk and dairy products and detection method thereof

The invention discloses a kit for detecting beta-lactamase in raw milk and dairy products and a detection method thereof, and reagents of the kit for detecting the beta-lactamase comprise a beta-lactamase standard product, a benzylpenicillin potassium standard liquid product, a protein precipitator,...

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Bibliographische Detailangaben
Hauptverfasser: XIE YANLI, ZHANG XINXIAO, CHEN YING, HAN XIAOXIAN, HUO QUANGONG, LI XUEQIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a kit for detecting beta-lactamase in raw milk and dairy products and a detection method thereof, and reagents of the kit for detecting the beta-lactamase comprise a beta-lactamase standard product, a benzylpenicillin potassium standard liquid product, a protein precipitator, namely trichloroacetic acid (TCA), a color developing agent, namely 2,2'-biquinoline ethanol solution and a complexing agent, namely CuSO4. The detection method comprises the following steps: firstly setting parameters of a detecting instrument, adding distilled water, standard liquid and a sampleinto a blank tube, a standard tube and a sample tube respectively, simultaneously adding the protein precipitator TCA, fully oscillating and mixing, then placing into a centrifuge for centrifugation at 4000rpm for 10 minutes, taking supernatant liquid, adding the 2,2'-biquinoline ethanol solution, fully uniformly shaking, standing at room temperature for 5 minutes, then performing colorimetric determination through the in