Method and kit for detecting isoniazid resistance mutation of Mycobacterium tuberculosis

The invention relates to techniques for detecting drug resistance mutation of Mycobacterium tuberculosis and provides a method and kit for detecting isoniazid resistance mutation of Mycobacterium tuberculosis, which have low cost and high throughout and are simple and specific. The method comprises...

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Bibliographische Detailangaben
Hauptverfasser:	LI QINGGE, HU SIYU, QUAN SHENGMAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to techniques for detecting drug resistance mutation of Mycobacterium tuberculosis and provides a method and kit for detecting isoniazid resistance mutation of Mycobacterium tuberculosis, which have low cost and high throughout and are simple and specific. The method comprises the following steps of: designing primers and probes by using the primer design software Primer Premier 5 according to the nucleic acid sequence of the H37Rv genome of Mycobacterium tuberculosis in the GenBank, extracting DNA of a Mycobacterium tuberculosis sample, constructing a PCR (polymerase chain reaction) system, analyzing PCR products by use of a melting curve after PCR is completed, and differentiating wild-type and mutation-type according to the differences in melting point and shape of the melting peaks. By adopting the real-time PCR melting curve, almost all the known drug resistance mutation types can be involved. Besides, the method and kit have the advantages of low cost, simplicity in operation, rapi