Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same
The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for plan...
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| creator | WU HAIYAN LI MINGSHU WANG HONGJUN WANG WEIWEI JIN RONGDE YU LEI LIU CHUNGUANG FAN ZUOWEI GAI JIAHUI LIU SHUXIA GAO XINGAI CHEN BAOYU |
| description | The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-diss |
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The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. 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The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. 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The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-diss</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERTILISERS FERTILISERS CHARACTERISED BY THEIR FORM MANUFACTURE THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05 MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same |
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