Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same

The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for plan...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WU HAIYAN, LI MINGSHU, WANG HONGJUN, WANG WEIWEI, JIN RONGDE, YU LEI, LIU CHUNGUANG, FAN ZUOWEI, GAI JIAHUI, LIU SHUXIA, GAO XINGAI, CHEN BAOYU
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-diss