Biocatalytic preparation of nucleosides

Biocatalytic preparation of nucleosides

A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at leas...

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Hauptverfasser: BODE MOIRA LEANNE, RASHAMUSE KONANANI JUSTIC, LOUW MAUREEN ELIZABETH, HENNESSY FRITHA, VISSER DANIEL FINSCH, GORDON GREGORY ERNEST ROBERT, BRADY DEAN
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Zusammenfassung:A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at least 90% sequence similarity thereto, and a pyhmidine nucleoside phosphorylase (PyNPase).