Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe
The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a m...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIN LI WEI BIWEN GAO HUAN ZHENG YI WU JIKUI ZHANG JUNLING |
| description | The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a mercuric ion identification component, a DNA double-strand intercalator SYBRGREENI is used as a fluorescence signal molecule, and then mercuric ion concentration in a sample to be detected is subjected to fluorescence detection on the basis of the difference of fluorescence signal intensity during the single-strand and double-strand binding between the SYBRGREENI and the prober containing the T-T mismatched DNA. The method disclosed by the invention has the advantages of no environmental pollution, good selectivity to mercuric ion, capability of eliminating interference from most of metal ions, low cost, high sensitivity, high detection speed and the like. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN102200510A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN102200510A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN102200510A3</originalsourceid><addsrcrecordid>eNqNjEsKwjAURTtxIOoengsopBUXUFrFiR1l5KSkr7c00HxI0v0bxQU4upzD4e6L1xOBt6CZtLPEzjJsCip9aF43FxCzYdCEBP5qg7S4iUYVMVFmWUoyOhqVeMmm6xvywY04FrtZrRGn3x6K8_0m20cJ7wZEr_Ix0tD2lahrIa6VaC7_NG8PeDpn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe</title><source>esp@cenet</source><creator>LIN LI ; WEI BIWEN ; GAO HUAN ; ZHENG YI ; WU JIKUI ; ZHANG JUNLING</creator><creatorcontrib>LIN LI ; WEI BIWEN ; GAO HUAN ; ZHENG YI ; WU JIKUI ; ZHANG JUNLING</creatorcontrib><description>The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a mercuric ion identification component, a DNA double-strand intercalator SYBRGREENI is used as a fluorescence signal molecule, and then mercuric ion concentration in a sample to be detected is subjected to fluorescence detection on the basis of the difference of fluorescence signal intensity during the single-strand and double-strand binding between the SYBRGREENI and the prober containing the T-T mismatched DNA. The method disclosed by the invention has the advantages of no environmental pollution, good selectivity to mercuric ion, capability of eliminating interference from most of metal ions, low cost, high sensitivity, high detection speed and the like.</description><language>chi ; eng</language><subject>ADHESIVES ; CHEMISTRY ; DYES ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE ; MEASURING ; METALLURGY ; MISCELLANEOUS APPLICATIONS OF MATERIALS ; MISCELLANEOUS COMPOSITIONS ; NATURAL RESINS ; PAINTS ; PHYSICS ; POLISHES ; TESTING</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110928&DB=EPODOC&CC=CN&NR=102200510A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110928&DB=EPODOC&CC=CN&NR=102200510A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIN LI</creatorcontrib><creatorcontrib>WEI BIWEN</creatorcontrib><creatorcontrib>GAO HUAN</creatorcontrib><creatorcontrib>ZHENG YI</creatorcontrib><creatorcontrib>WU JIKUI</creatorcontrib><creatorcontrib>ZHANG JUNLING</creatorcontrib><title>Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe</title><description>The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a mercuric ion identification component, a DNA double-strand intercalator SYBRGREENI is used as a fluorescence signal molecule, and then mercuric ion concentration in a sample to be detected is subjected to fluorescence detection on the basis of the difference of fluorescence signal intensity during the single-strand and double-strand binding between the SYBRGREENI and the prober containing the T-T mismatched DNA. The method disclosed by the invention has the advantages of no environmental pollution, good selectivity to mercuric ion, capability of eliminating interference from most of metal ions, low cost, high sensitivity, high detection speed and the like.</description><subject>ADHESIVES</subject><subject>CHEMISTRY</subject><subject>DYES</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MISCELLANEOUS APPLICATIONS OF MATERIALS</subject><subject>MISCELLANEOUS COMPOSITIONS</subject><subject>NATURAL RESINS</subject><subject>PAINTS</subject><subject>PHYSICS</subject><subject>POLISHES</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2011</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEsKwjAURTtxIOoengsopBUXUFrFiR1l5KSkr7c00HxI0v0bxQU4upzD4e6L1xOBt6CZtLPEzjJsCip9aF43FxCzYdCEBP5qg7S4iUYVMVFmWUoyOhqVeMmm6xvywY04FrtZrRGn3x6K8_0m20cJ7wZEr_Ix0tD2lahrIa6VaC7_NG8PeDpn</recordid><startdate>20110928</startdate><enddate>20110928</enddate><creator>LIN LI</creator><creator>WEI BIWEN</creator><creator>GAO HUAN</creator><creator>ZHENG YI</creator><creator>WU JIKUI</creator><creator>ZHANG JUNLING</creator><scope>EVB</scope></search><sort><creationdate>20110928</creationdate><title>Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe</title><author>LIN LI ; WEI BIWEN ; GAO HUAN ; ZHENG YI ; WU JIKUI ; ZHANG JUNLING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN102200510A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2011</creationdate><topic>ADHESIVES</topic><topic>CHEMISTRY</topic><topic>DYES</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MISCELLANEOUS APPLICATIONS OF MATERIALS</topic><topic>MISCELLANEOUS COMPOSITIONS</topic><topic>NATURAL RESINS</topic><topic>PAINTS</topic><topic>PHYSICS</topic><topic>POLISHES</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>LIN LI</creatorcontrib><creatorcontrib>WEI BIWEN</creatorcontrib><creatorcontrib>GAO HUAN</creatorcontrib><creatorcontrib>ZHENG YI</creatorcontrib><creatorcontrib>WU JIKUI</creatorcontrib><creatorcontrib>ZHANG JUNLING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIN LI</au><au>WEI BIWEN</au><au>GAO HUAN</au><au>ZHENG YI</au><au>WU JIKUI</au><au>ZHANG JUNLING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe</title><date>2011-09-28</date><risdate>2011</risdate><abstract>The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a mercuric ion identification component, a DNA double-strand intercalator SYBRGREENI is used as a fluorescence signal molecule, and then mercuric ion concentration in a sample to be detected is subjected to fluorescence detection on the basis of the difference of fluorescence signal intensity during the single-strand and double-strand binding between the SYBRGREENI and the prober containing the T-T mismatched DNA. The method disclosed by the invention has the advantages of no environmental pollution, good selectivity to mercuric ion, capability of eliminating interference from most of metal ions, low cost, high sensitivity, high detection speed and the like.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN102200510A |
| source | esp@cenet |
| subjects | ADHESIVES CHEMISTRY DYES INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS PAINTS PHYSICS POLISHES TESTING |
| title | Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A37%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIN%20LI&rft.date=2011-09-28&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN102200510A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |