Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe

The invention relates to the field of mercuric ion concentration detection and analysis and particularly discloses a mercuric ion concentration fluorescence detection method based on a T-T mismatched DNA probe. The method is characterized in that a prober containing T-T mismatched DNA is used as a mercuric ion identification component, a DNA double-strand intercalator SYBRGREENI is used as a fluorescence signal molecule, and then mercuric ion concentration in a sample to be detected is subjected to fluorescence detection on the basis of the difference of fluorescence signal intensity during the single-strand and double-strand binding between the SYBRGREENI and the prober containing the T-T mismatched DNA. The method disclosed by the invention has the advantages of no environmental pollution, good selectivity to mercuric ion, capability of eliminating interference from most of metal ions, low cost, high sensitivity, high detection speed and the like.