Method for extracting superoxide dismutase (SOD) from porcine blood red cells

The invention relates to a method for extracting superoxide dismutase (SOD) from porcine blood red cells, in the method, anticoagulation treatment is adopted for preventing blood coagulation and breaking of blood cells. Colloid mill breaking and low-temperature swelling are adopted for releasing mat...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG LIJUAN, SHEN FENG, WANG CHANGWEI, XIA JIHUA, SONG GUQUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for extracting superoxide dismutase (SOD) from porcine blood red cells, in the method, anticoagulation treatment is adopted for preventing blood coagulation and breaking of blood cells. Colloid mill breaking and low-temperature swelling are adopted for releasing matters dissolved in the blood cells to the maximum extent so as to get hemolysate. Thermal change is performed on the hemolysate twice for removing impurities, removing hemoglobin and a large number of impurity proteins so as to get SOD crude enzyme liquid, and ultrafiltration concentration, gradient elution, dialysis, salt removal and freeze-drying are further performed on the SOD crude enzyme liquid so as to get an SOD product. The method is simple to operate and easy to master; furthermore, by adopting the method, the product quality can be effectively improved, the production cost can be reduced, the production cycle can be shortened, the production efficiency can be improved, and the method is applicable to indu