Method for extracting immunoglobulin A (IgA) of Przewalski's horse noninvasively

The invention discloses a method for extracting immunoglobulin A (IgA) of Przewalski's horse noninvasively. The method for extracting animal immunoglobulin comprises the following steps of: mixing animal excrement and extraction buffer to obtain a mixture; and centrifuging the mixture to obtain supernatant, namely immunoglobulin in the animal, wherein the extraction buffer consists of phosphate buffered saline (PBS) and Tween-20. The method for extracting the immunoglobulin of the Przewalski's horse noninvasively is simple and high-efficiency. In the method, the fresh excrement of the Przewalski's horse is collected and dissolved in the extraction buffer added with the Tween-20 at certain concentration, and the supernatant is obtained through two-step centrifugation, namely the high-concentration immunoglobulin of the Przewalski's horse can be extracted. The method is easy to operate and high in extraction efficiency.