Method for filtering nucleic acids, in particular from fixed tissue

The present invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention, and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is he...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HENNIG GUIDO, EUTING HEIKE, BOHMANN KERSTIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The present invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention, and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.